Genetic and physical evidence for plasmid control of Shigella sonnei form I cell surface antigen
نویسندگان
چکیده
منابع مشابه
Molecular cloning and characterization of form I antigen genes of Shigella sonnei.
Sau3AI-generated DNA fragments of the Shigella sonnei large plasmid encoding the form I antigen were cloned into Escherichia coli with cosmid vector pHSG262. One resulting plasmid, designated pJK1137, was studied further. Restriction endonuclease mapping and analysis of transposon Tn3 insertion mutants demonstrated that the form I antigen genes were located within a region of about 12.6 kb cons...
متن کاملStudy of paracolon organisms with the major antigen of Shigella sonnei, form I.
Ferguson and co-workers (Ferguson and Henderson, 1947; Ferguson et al., 1947) reported the isolation of a motile, anaerogenic paracolon organism which they designated C 27, sharing the somatic antigen with Shigella sonnei, form I. A similar strain was isolated by Wheeler (personal communication, 1947). In Ceylon, the first strain of this type was isolated during an investigation of the carrier ...
متن کاملIdentification of Shigella sonnei form I plasmid genes necessary for cell invasion and their conservation among Shigella species and enteroinvasive Escherichia coli.
A series of Tn1 insertions in pSS120, the 120-megadalton form I plasmid of Shigella sonnei, were constructed by a Tn1-mediated conduction system previously described (H. Watanabe and A. Nakamura, Infect. Immun. 48:260-262, 1985, and screened for cell invasion in a tissue culture assay. The analysis of Tn1 insertion sites of seven noninvasive mutants suggested that four separate HindIII fragment...
متن کاملShigella sonnei plasmids: evidence that a large plasmid is necessary for virulence.
Virulent form I Shigella sonnei strains contain a 120-megadalton plasmid that is absent in their form II derivatives, which are always avirulent and devoid of O side chains. In the present study, 165 biochemical and antibiotic traits were assessed, but no experimentally useful phenotype could be associated with this large form I plasmid. Therefore, the form I plasmids of several S. sonnei strai...
متن کاملAn O Antigen Capsule Modulates Bacterial Pathogenesis in Shigella sonnei
Shigella is the leading cause for dysentery worldwide. Together with several virulence factors employed for invasion, the presence and length of the O antigen (OAg) of the lipopolysaccharide (LPS) plays a key role in pathogenesis. S. flexneri 2a has a bimodal OAg chain length distribution regulated in a growth-dependent manner, whereas S. sonnei LPS comprises a monomodal OAg. Here we reveal tha...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1980
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.29.1.207-214.1980